Search results for "MELTING CURVE"

showing 10 items of 14 documents

Validating a Rapid Method for Detecting Common Polymorphisms in the APOA5 Gene by Melting Curve Analysis Using LightTyper

2005

The recently identified apolipoprotein A-V gene (APOA5) has been shown to play an important role in hypertriglyceridemia (1). Genetic variation in APOA5 has been consistently associated with plasma triglyceride concentrations in several studies (2)(3)(4). Moreover, some studies have demonstrated additional associations with lipoprotein subclasses, remnant-like particles, and cardiovascular disease risk (4)(5)(6). Several single-nucleotide polymorphisms (SNPs) in the human APOA5 gene have been detected with differing frequencies depending on the population analyzed (7)(8), and Klos et al.(7) have also suggested context-dependent associations in different populations. Overall, 5 common SNPs, …

AdultNonsynonymous substitutionAdolescentGenotypeClinical BiochemistryPopulationSingle-nucleotide polymorphismBiologyPolymerase Chain ReactionMelting curve analysisHumanseducationGenotypingApolipoproteins AAgedGeneticseducation.field_of_studyPolymorphism GeneticBiochemistry (medical)HaplotypeMiddle AgedSNP genotypingApolipoproteinsApolipoprotein A-VRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthClinical Chemistry
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Schmelz- und kristallisationserscheinungen bei makromolekularen substanzen. V. Partielles schmelzen und neukristallisieren von terylen

1960

Das nach dem Erhitzen oft innerhalb von Sekunden stattfindende partielle Schmelzen und erneute Kristallisieren von Terylen konnte mittels einer Abschreckmethode quantitativ untersucht werden. Die je nach der thermischen Vorbehandlung verschiedenen Schmelzkurven wurden unbeeinflust von Neukristallisationseffekten aufgenommen und weiterhin der Nachweis erbracht, das nur die gesamte Schmelzkurve und nicht der Kristallisationsgrad allein den kristallinen Zustand einer Probe ausreichend charakterisiert. Mikroskopische Untersuchungen zeigten, das partielles Schmelzen und Neukristallisation innerhalb der Spharolithe stattfinden. Weiterhin wurde die Kristallisation beim Abkuhlen von partiell geschm…

CrystallographylawChemistryPolymer chemistryPartial meltingBiophysicsCrystallizationMelting curve analysislaw.inventionDie Makromolekulare Chemie
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A real-time PCR assay for detection and quantification of 2-branched (1,3)-β-D–glucan producing lactic acid bacteria in cider

2010

28 p.-1 fig.-4 tab.

DNA Bacterialbeta-GlucansFood spoilageMicrobiologyMelting curve analysisMicrobiologyPolysaccharidesLactobacillus(13)(12)--D-glucanLactic acid bacteriaFood sciencePediococcusOenococcusOenococcus oeniDNA PrimersbiologyBacteriaSpoilageReverse Transcriptase Polymerase Chain ReactionAlcoholic BeveragesGeneral MedicineAmpliconbiology.organism_classificationBacterial Typing TechniquesLactobacillusCidersGenes BacterialGlucosyltransferasesFood MicrobiologyPediococcusProteoglycansOenococcusBacteriaFood ScienceReal-time PCR
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Melting of tantalum at high pressure determined by angle dispersive x-ray diffraction in a double-sided laser-heated diamond-anvil cell

2003

The high pressure and high temperature phase diagram of Ta has been studied in a laser-heated diamond-anvil cell (DAC) using x-ray diffraction measurements up to 52 GPa and 3800 K. The melting was observed at nine different pressures, being the melting temperature in good agreement with previous laser-heated DAC experiments, but in contradiction with several theoretical calculations and previous piston-cylinder apparatus experiments. A small slope for the melting curve of Ta is estimated (dTm/dP = 24 K/GPa at 1 bar) and a possible explanation for this behaviour is given. Finally, a P-V-T equation of states is obtained, being the temperature dependence of the thermal expansion coefficient an…

DiffractionBulk modulusCondensed Matter - Materials ScienceMaterials scienceTantalumAnalytical chemistryMaterials Science (cond-mat.mtrl-sci)FOS: Physical scienceschemistry.chemical_elementCondensed Matter PhysicsMelting curve analysisThermal expansionDiamond anvil cellchemistryCondensed Matter::SuperconductivityX-ray crystallographyGeneral Materials SciencePhase diagram
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In situ characterization of the high pressure – high temperature melting curve of platinum

2019

AbstractIn this work, the melting line of platinum has been characterized both experimentally, using synchrotron X-ray diffraction in laser-heated diamond-anvil cells, and theoretically, using ab initio simulations. In the investigated pressure and temperature range (pressure between 10 GPa and 110 GPa and temperature between 300 K and 4800 K), only the face-centered cubic phase of platinum has been observed. The melting points obtained with the two techniques are in good agreement. Furthermore, the obtained results agree and considerably extend the melting line previously obtained in large-volume devices and in one laser-heated diamond-anvil cells experiment, in which the speckle method wa…

DiffractionMaterials scienceAb initioAnalytical chemistrylcsh:Medicinechemistry.chemical_element02 engineering and technology01 natural sciencesMelting curve analysisArticlelaw.inventionlawPhase (matter)0103 physical scienceslcsh:Science010306 general physicsMultidisciplinarylcsh:RAtmospheric temperature range021001 nanoscience & nanotechnologySynchrotronMaterials sciencePhase transitions and critical phenomenachemistryMelting pointlcsh:Q0210 nano-technologyPlatinumScientific Reports
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Melting curve and phase diagram of vanadium under high-pressure and high-temperature conditions

2019

Melting curve and phase diagram of vanadium under high-pressure and high-temperature conditions We report a combined experimental and theoretical study of the melting curve and the structural behavior of vanadium under extreme pressure and temperature. We performed powder x-ray-diffraction experiments up to 120 GPa and 4000 K, determining the phase boundary of the body-centered cubic-to-rhombohedral transition and melting temperatures at different pressures. Melting temperatures have also been established from the observation of temperature plateaus during laser heating, and the results from the density-functional theory calculations. Results obtained from our experiments and calculations a…

DiffractionPhase boundaryEquation of stateMaterials scienceThermodynamicsVanadiumchemistry.chemical_element02 engineering and technology01 natural sciencesMelting curve analysisCrystalCondensed Matter::Materials ScienceX-RAY-DIFFRACTIONNACLCondensed Matter::Superconductivity0103 physical sciencesELEMENTSCELL010306 general physicsTANTALUMPhase diagramCRYSTALIRON021001 nanoscience & nanotechnologyEQUATION-OF-STATEchemistryX-ray crystallographyCondensed Matter::Strongly Correlated Electrons0210 nano-technologySYSTEM
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High-pressure/high-temperature phase diagram of zinc

2018

The phase diagram of zinc (Zn) has been explored up to 140 GPa and 6000K, by combining optical observations, x-ray diffraction, and ab initio calculations. In the pressure range covered by this study, Zn is found to retain a hexagonal close-packed (hcp) crystal symmetry up to the melting temperature. The known decrease of the axial ratio (c/a) of the hcp phase of Zn under compression is observed in x-ray diffraction experiments from 300K up to the melting temperature. The pressure at which c/a reaches root 3 (approximate to 10GPa) is slightly affected by temperature. When this axial ratio is reached, we observed that single crystals of Zn, formed at high temperature, break into multiple pol…

DiffractionPhase transitionMaterials sciencemeltingPOWDER DIFFRACTIONELECTRONIC TOPOLOGICAL TRANSITIONSThermodynamicschemistry.chemical_elementFOS: Physical sciences02 engineering and technologyCrystal structureZincDIAMOND-ANVIL CELL01 natural scienceshigh temperatureCondensed Matter::Materials ScienceX-RAY-DIFFRACTIONPhase (matter)Condensed Matter::Superconductivity0103 physical sciencesGeneral Materials Science010306 general physicsMELTING CURVEPhase diagramCondensed Matter - Materials ScienceAxial ratioSYNCHROTRONab initio calculationszincMaterials Science (cond-mat.mtrl-sci)021001 nanoscience & nanotechnologyCondensed Matter PhysicsCompression (physics)EQUATION-OF-STATEhigh pressurechemistryx-ray diffractionphase transitionZNMETALS0210 nano-technologyRESISTANCE
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Microscopic evidence of a flat melting curve of tantalum

2010

International audience; New data on the high-pressure melting curve of Ta up to 48GPa are reported. Evidence of melting from changes in sample texture was found in five different experiments using scanning electron microscopy. The obtained melting temperatures are in excellent agreement with earlier measurements using x-ray diffraction or the laser-speckled method but are in contrast with several theoretical calculations. The results are also compared with shock-wave data. These findings are of geophysical relevance because they confirm the validity of earlier experimental techniques that resulted in low melting slopes of the transition metals measured in the diamond-anvil cell, including i…

Diffractionlaser-heatingMaterials sciencehigh-pressurePhysics and Astronomy (miscellaneous)Scanning electron microscope62.50.-p64.70.djTantalumAnalytical chemistrychemistry.chemical_element02 engineering and technology01 natural sciencesdiamond-anvil cellDiamond anvil cellMelting curve analysistransition metalsTransition metal0103 physical sciencesTexture (crystalline)010306 general physics62.50.EfAstronomy and Astrophysics021001 nanoscience & nanotechnologyCrystallographyGeophysicsmelting curvechemistrySpace and Planetary Science0210 nano-technologyMelting-point depression
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Ab Initio Phase Diagram of Chromium to 2.5 TPa

2022

Chromium possesses remarkable physical properties such as hardness and corrosion resistance. Chromium is also a very important geophysical material as it is assumed that lighter Cr isotopes were dissolved in the Earth’s molten core during the planet’s formation, which makes Cr one of the main constituents of the Earth’s core. Unfortunately, Cr has remained one of the least studied 3d transition metals. In a very recent combined experimental and theoretical study (Anzellini et al., Scientific Reports, 2022), the equation of state and melting curve of chromium were studied to 150 GPa, and it was determined that the ambient body-centered cubic (bcc) phase of crystalline Cr re…

Fluid Flow and Transfer ProcessesProcess Chemistry and TechnologyGeneral EngineeringFísicaGeneral Materials ScienceQuímicaquantum molecular dynamics; melting curve; solid–solid phase transition boundary; equation of state; multi-phase materialsInstrumentationMaterialsComputer Science Applications
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Concordance of assays designed for the quantification of JAK2V617F: a multicenter study

2008

Background Many different techniques have been designed for the quantification of JAK2 V617F allelic burden, sometimes producing discrepant results. Design and Methods JAK2 V617F quantification techniques were compared among 16 centers using 11 assays based on quantitative polymerase chain reaction (with mutation-specific primers or probes, or fluorescent resonance energy transfer/melting curve analysis), allele-specific polymerase chain reaction, conventional sequencing or pyrosequencing. Results A first series of blinded samples (granulocyte DNA, n=29) was analyzed. Seven assays (12 centers) reported values inside the mean±2SD; the mean coefficient of variation was 31%. Sequencing techniq…

MaleSerial dilutionPhenylalanineCoefficient of variationBiologyMelting curve analysislaw.inventionlawhemic and lymphatic diseasesTaqManHumansAllelesPolymerase chain reactionValineOriginal ArticlesHematologyJanus Kinase 2Molecular biologyPedigreePhenotypeReal-time polymerase chain reactionMutationPyrosequencingFemalePrimer (molecular biology)Thrombocythemia EssentialHaematologica
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